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And functions of biological membranes
To observe structure of a membrane in a usual optical microscope it is impossible. That it to understand, we shall recollect, that is permitting limit of device Z. This minimal distance between two points, which images else can be seen separate. It is natural, that the it is less Z, the more qualitatively the device, as allows seeing smaller structures. For a rough estimate of the permitting ability of a n optical microscope we use parity: 
.
Instead of 
let's substitute in the formula the minimal value of length of a wave of visible light(
), we shall receive for a permitting limit Z=200 nanometer. This size approximately in 20 times more thickness of a membrane, therefore about its supervision in an optical microscope cannot be speeches. But use of a microprojection and a microphoto is possible. Formation of the microscopic image occurs to participation of the person and comes to the end with formation of the valid image in an eye. The usual microscope does not create the valid image, however for photographing (microphoto) or projections of the microscopic image to the screen (microprojection) the valid image should be received. For this purpose the image given by objective Ob, it is necessary to arrange further of focal length of eyepiece Ep (fig.1).
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